Involvement of Wheat Germ Agglutinin (WGA)-Binding Protein in the Induction of the Acrosome Reaction of the Sea Urchin, Strongylocentrotus intermedius. I. WGA Affects the Ion Fluxes Associated with the Acrosome Reaction. (sea urchin/acrosome reaction/wheat germ agglutinin/ion flux/species specificity)

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Abstract
The lectin wheat germ agglutinin (WGA) inhibited the egg jelly-induced acrosome reaction (AR) of sperm of the sea urchin, Strongylocentrotus intermedius. Fluorescein-conjugated WGA applied to sperm bound to the acrosomal region, to the midpiece, and to the tip of the flagellum. These effects were not observed in the presence of N-acetyl-D-glucosamine. When the egg jelly was replaced by artificial AR inducers such as A23187 or nigericin, the AR was not inhibited by WGA. Results obtained using a Ca2+ indicator fura-2, a pH indicator 2'',7''-bis(carboxyethyl)carboxylfuorescein (BCECF) and a membrane potential sensitive dye 3,3''-dipentyl 2,2''-dioxacarbocyanine [diO-C5(3)] showed that WGA suppresses the egg jelly-induced influx of Ca2+ and slightly suppresses the efflux of H+ caused by the egg jelly, whereas the depolarization of the plasma membrane by the egg jelly is remarkably amplified by the treatment with WGA. These results suggest that WGA affects the regulatory system of the ion fluxes associated with the AR. The target protein of WGA (WGA-binding protein) was a membrane glycoprotein of 260 kD under non-reducing condition.