Lack of Correlation Between the Immunobead Test and the Enzyme‐Linked Immunosorbent Assay for Sperm Antibody Detection

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Abstract
A total of 41 sera submitted for routine sperm antibody screening were tested by both the indirect immunobead test (IBT) against viable sperm as antigen, and an enzyme-linked immunosorbent assay (ELISA) using the supernatant fraction from washed, sonicated sperm as antigen. Sixteen sera were positive by either IBT or ELISA, but none were positive by both tests. It was concluded that the tests may be detecting antibodies reacting with distinct antigen subgroups, possibly because the ELISA may be detecting antibodies directed against internal antigens or non-sperm-specific antigens such as HLA.