GROWTH-INHIBITION OF MALIGNANT HYPER-NEPHROMA CELLS BY AUTOLOGOUS LYSOPHOSPHOLIPID INCUBATED MACROPHAGES OBTAINED BY A NEW METHOD
- 1 January 1981
- journal article
- research article
- Vol. 1 (3) , 135-+
Abstract
To obtain pure human macrophages, mononuclear cells from peripheral blood were cultured on teflon membranes and the non-adherent lymphocytes removed. After 24 h, all remaining adherent cells were detached from the membranes with 100% viability. They showed all the morphological and cytochemical characteristics of human monocytes. Within 10 days of cultivation they differentiated into monolayers of pure macrophages. Untreated macrophages of this origin showed only limited cytostatic effects on autologous [human] hypernephroma cells in vitro. After preincubation with different [antineoplastic] alkyl-lysophospholipids [racemic-1-octadecyl-2-methyl-sn-glycero-3-phosphocholine and racemic-1-octadecyl-sn-glycero-3-phosphocholine] they revealed a tumor growth inhibition capability of up to 90%.This publication has 9 references indexed in Scilit:
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