Transcript profiles ofNitrosomonas europaeaduring growth and upon deprivation of ammonia and carbonate
Open Access
- 1 April 2006
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 257 (1) , 76-83
- https://doi.org/10.1111/j.1574-6968.2006.00152.x
Abstract
The transcriptome of Nitrosomonas europaea was analyzed with whole-genome microarrays. Growing cells were compared to cells deprived of (NH4)2SO4 and Na2CO3. Hybridization signals were detected for 76% of the genes represented on the array under either or both conditions. Transcript levels for 68% of the genes were at least twofold higher in growing cells than in deprived cells, while only 0.42% of the genes were present at more than twofold higher levels in deprived cells. Transcript levels for the remaining 7% of the genes did not change significantly with the treatments. These trends were confirmed for selected genes by Northern hybridizations and quantitative RT-PCR. Compared to heterotrophic bacteria, N. europaea downregulates a greater proportion of its genes and fewer genes appear to be associated with the adaptation to starvation.Keywords
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