The development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses.

  • 1 January 1981
    • journal article
    • research article
    • Vol. 50, 103-10
Abstract
Two high productivity monolayer culture methods, the microcarrier and glass sphere culture, were compared for their capacity to support the growth of MRC-5 and Vero cells, and Herpes simplex type 2 virus. Cell growth was similar in both systems giving yields of 14 X 10(5)/cm2 in microcarrier and 18 X 10(5)/cm2 in glass sphere cultures with yields of Vero cells being marginally better than MRC-5 cells. Virus yields were only slightly lower in these cultures than in small scale stationary cultures and confirmed the fact that MRC-5 cells produce twice as much HSV-2 as Vero cells, thus neutralising the growth advantage of these cells. Techniques for harvesting the virus in small volumes need to be improved in order to get a high titre suspension from the high capacity cultures.

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