Accelerated immunohistochemical staining by microwaves
- 1 August 1990
- journal article
- research article
- Published by Wiley in The Journal of Pathology
- Vol. 161 (4) , 327-334
- https://doi.org/10.1002/path.1711610409
Abstract
The application of microwave irradiation is extended to include the acceleration of the streptavidin‐biotin peroxidase staining procedure for the detection of a wide range of antigens in paraffin‐embedded sections of both normal and neoplastic tissues. Microwave irradiation is used for all procedural steps requiring incubation so that the entire staining procedure can be completed within 20 min. The technique is simple and convenient, with uniform irradiation times for different tissue antigens. The results obtained equalled that of conventional staining procedures.Keywords
This publication has 9 references indexed in Scilit:
- Antigen preservation in microwave‐irradiated tissues: A comparison with formaldehyde fixationThe Journal of Pathology, 1988
- Microwave stimulation of an immunological reaction (CEA/anti-CEA) and its use in immunohistochemistryJournal of Molecular Histology, 1988
- Rapid immunofluorescence staining of human renal biopsy specimens using microwave irradiation.Journal of Clinical Pathology, 1987
- A method of rapid fixation of large biopsy specimens using microwave irradiationPathology, 1986
- Ultrafast microwave energy fixation for electron microscopy.Journal of Histochemistry & Cytochemistry, 1986
- Microwave‐stimulated diffusion for fast processing of tissue: reduced dehydrating, clearing, and impregnating timesHistopathology, 1986
- Rapid immunoperoxidase staining of lymphocyte antigens using microwave irradiationThe Journal of Pathology, 1986
- Microwave irradiation as a form of fixation for light and electron microscopyThe Journal of Pathology, 1985