The int genes of bacteriophages P22 and λ are regulated by different mechanisms
- 1 July 1993
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 9 (2) , 261-271
- https://doi.org/10.1111/j.1365-2958.1993.tb01688.x
Abstract
Bacteriophage P22 and λ are related bacteriophages with similar gene organizations. In λ the cII‐dependent PI promoter is responsible for λint gene expression. The only apparent counterpart to PI in P22 is oriented in the opposite direction, and cannot transcribe the P22 int gene. We show that this promoter, called Pal, is active both in vivo and in vitro, and is dependent upon the P22 cII‐like gene, called c1. We have also determined the DNA sequence of a 3.3 kb segment that closes the gap between previously reported sequences to give a continuous sequence between the P22 pL promoter and the int gene. The newly determined sequence is densely packed with genes from the pL direction, and the proteins predicted by the sequence show excellent correlation with the proteins mapped by Youderian and Susskind in 1980. However, the sequence contains no apparent genes in the opposite (pal) direction, and no additional binding motifs for the P22 c1 protein. We conclude that int gene expression in P22 is regulated by a different mechanism than in λ.Keywords
This publication has 49 references indexed in Scilit:
- RNase III-dependent hydrolysis of lambda cII-O gene mRNA mediated by lambda OOP antisense RNA.Genes & Development, 1990
- Genetic structure of the bacteriophage P22 PL operonJournal of Molecular Biology, 1989
- Specific endonucleolytic cleavage sites for decay of Escherichia coli mRNAJournal of Molecular Biology, 1986
- Structural and regulatory divergence among site-specific recombination genes of lambdoid phageJournal of Molecular Biology, 1986
- Bacteriophage P22 Cro protein: sequence, purification, and propertiesBiochemistry, 1986
- Sequence analysis of a region from the early right operon in phage P22 including the replication genes 18 and 12Gene, 1984
- Removal of a terminator structure by RNA processing regulates int gene expressionJournal of Molecular Biology, 1984
- Bacteriophage λ protein cII binds promoters on the opposite face of the DNA helix from RNA polymeraseNature, 1983
- Control of lysogenization by phage P22 I. The P22 cro geneJournal of Molecular Biology, 1981
- A genetic analysis of the att-int-xis region of coliphage lambdaJournal of Molecular Biology, 1977