The Application of HPLC Methodology for the Analysis of Receptor Mediated Changes in Phosphoinositide Metabolism
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Receptor Research
- Vol. 4 (1-6) , 505-520
- https://doi.org/10.3109/10799898409042570
Abstract
HPLC methodology has found wide application in analytical problems in biochemistry. To study the metabolism of phosphatidylinositol and its regulation by receptor mediated events, HPLC could be a valuable technique. It has been recently demonstrated that a variety of hormones and neurotransmittors act to stimulate hydrolysis of phosphoinositides by a phospholipase C. To monitor this reaction, we have analysed the formation of radiolabelled inositol phosphates from phosphoinositides. The present paper describes a rapid HPLC procedure, to separate inositol phosphates from myo-inositol, which could be used in pharmacological studies of receptors linked to phosphoinositide hydrolysis. The potential of the application of HPLC to the analysis of the phospholipids involved is discussed.Keywords
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