Non-plasmid associated transfer of antibiotic resistance in Bacteroides
- 1 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 8 (suppl D) , 77-86
- https://doi.org/10.1093/jac/8.suppl_d.77
Abstract
Resistance to tetracycline and clindamycin was transferred from a strain of Bacteroides fragilis (V503) to a plasmidless strain of Bacteroides uniformis (V528) during in-vitro filter matings. Transfer was dependent on cell-to-cell contact but was insensitive to deoxyribonuclease and was not mediated by chloroform- or filtersterilized donor broth cultures. A 3.7 × 10 6 dalton plasmid present in B. fragilis V503 was not associated with either the drug resistance or conjugal transfer properties. No additional species of plasmid DNA could be associated with the observed transferable resistances. Furthermore, the resistance markers of B. fragilis V503 could not be eliminated with known plasmid-curing regimens. B. fragilis V503 was found to contain DNA sequences that shared homology with the clindamycin resistance determinant of the well characterized Bacteroides plasmid, pBF4.Keywords
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