Präparative Isolierung von Mono- und Dipyrimidinnucleotiden aus depurinierter Heringsspermen-DNA

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Abstract
The preparative isolation of DNA fragments with defined sequences from hydrolysates of naturally occurring DNA was proposed as an alternative to their complicated synthesis and was partially realized. Starting with herring sperm DNA, which was depurinated in 100 g batches, one obtains 35-40 g of a pyrimidine-oligonucleotide mixture which is separated into high and low MW fragments via column chromatography on DEAE cellulose. The low MW pyrimidine-nucleotide mixture thus obtained is then selectively separated on a preparative scale into fragments with 1 and 2 monomer units by means of a 3-step separation procedure on QAE-Sephadex.