A Physiologically Active Principle from Cannabis sativa (Marihuana)

Abstract
A. crystalline substance, cannin, was isolated from the alcoholic extract of the crude drug and found physiologically active in dogs. The alcohol extract of the crude drug was diluted with water to 70% alcohol, and partitioned in petroleum ether. Salt forming compounds were extracted and colored substances adsorbed on ZnCO3. An active fraction was obtained by fractional precipitation from methanol with water. The product was fractionally distilled under 0.005 mm. pressure, the most active fraction being a reddish oil coming over at 128[degree]-135[degree]C. Crystals were obtained by cooling a solution of this oil in a methanol acetic acid mixture. After several recrystallizations from methanol, colorless needles obtained, M. P. 128[degree]-129[degree]C.

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