Unusual branch point selection involved in splicing of the alternatively processed Calcitonin/CGRP-I pre-mRNA
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (20) , 9513-9526
- https://doi.org/10.1093/nar/16.20.9513
Abstract
To study splice site selection in alternative RNA processing we used the human Calcitonin \ CGRP-I (CALC-I) gene. Expression of the CALC-I gene in thyroid C-cells results predominantly in calcitonin (CT) mRNA (containing exons 1 to 4) whereas CGRP-1 mRNA (containing exons 1,2,3,5 and 6) is the exclusive product in particular nerve cells. We previously reported that a model precursor RNA containing the exon 3 to exon 5 region is predominantly processed into CGRP-I mRNA in vitro using nuclear extracts of three different cell types. To study CT specific processing in Hela cell nuclear extracts we have used precursor RNAs corresponding to the exon 3 to exon 4 region containing only CT specific processing signals. The results revealed the usage of a uridine residue 23 nucleotides upstream of the 3'' splice site as the major site of lariat formation in CT specific splicing. The implications of this finding for the alternative, tissue specific processing of the CALC-I pre-mRNA and for branch point selection in general are discussed.This publication has 54 references indexed in Scilit:
- 5′ cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5′ splice region, not by the conserved 5′ GUCell, 1987
- Splicing of SV40 early pre-mRNA to large T and small t mRNAs utilizes different patterns of lariat branch sitesCell, 1987
- Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNACell, 1987
- Splice commitment dictates neuron-specific alternative RNA processing in calcitonin/CGRP gene expressionCell, 1987
- Structure and expression of the human calcitonin/CGRP genesFEBS Letters, 1986
- A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoproteinCell, 1986
- Effect of mutations at the lariat branch acceptor site on β-globin pre-mRNA splicing in vitroNature, 1986
- A compensatory base change in U1 snRNA suppresses a 5′ splice site mutationCell, 1986
- Cryptic branch point activation allows accurate in vitro splicing of human β-globin intron mutantsCell, 1985
- Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processingNature, 1983