Production of toxic shock syndrome toxin 1 by Staphylococcus aureus as determined by tampon disk-membrane-agar method

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Abstract
The influence of 17 commercially available tampons on production of toxic shock syndrome toxin 1 (TSST-1) by Staphyloccous aureus was investigated by using a tampon disk method. Filter membranes overlaying agar medium (with or without blood) in small petri dishes were spread inoculated with a TSST-1 producing strain of S. aureus. Disks cut from unrolled tampons were pressed and laid on the inoculated membranes; incubation for 19 h at 37.degree. C with 5% CO2 in air. CFU on the membranes and in the disks were enumerated, and the presence of TSST-1 in the disks and in the agar layers was determined. Tampons made of different materials supported characteristic levels of cell growth and toxin production in the tampon. Colonization of the interface surface of the tampon disks was heavy. The number of CFU extracted from the tampon disk ranged from 5 .times. 1010 to 82 .times. 1010. There was little variation in the CFU recovered from the membranes ([1.9 .+-. 0.4] .times. 1011). Sixty to 170 .mu.g of TSST-1 was recoverable from the agar, with an additional 10 to 90 .mu.g recoverable from tampon disks, depending on the type of tampon disk. The amount of toxin in the agar layer from the various tampon disks was relatively constant and indicated an important contribution of toxin from vaginal S. aureus cells not growing in the tampon. The main role of tampons in toxic shock syndrome may be that of providing a fibrous surface for heavy colonization and sufficient air for TSST-1 production.