FERTILITÉ ET PROLIFICITÉ DE TRUIES INSÉMINÉES AVEC DU SPERME CONGELÉ

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Abstract
Three techniques for preparation of semen before freezing were studied to examine the fertilizing capacity of spermatozoa. Whole ejaculates of 9 boars were divided into 3 fractions (A, B, C) and centriguged at 800 g for 15 mn at 30.degree. C. The precipitate was diluted with an unglycerolated glucose-egg-yolk diluent followed by cooling of the semen at 2 rates: group A 30.degree. C to 15.degree. C within I h and 15.degree. C within I h; groups B and C 30.degree. C to 15.degree. C within I h, holding at 15.degree. C for 4 h and cooling to 5.degree. C within I h. The semen was rediluted with the same diluent, but with glycerol at 15.degree. C after 1 h of cooling for groups A and B and 5 h of cooling for group C. The semen was frozen in pellets of 0.1 ml. Insemination doses were thawed in the diluent at 50.degree. C (1:5). Nulliparous sows (63) were inseminated with 3-4 .cntdot. 109 motile spermatozoa 34 h after the presumed onset of heat. Holding the semen for 4 h at 15.degree. C significantly improved (P .ltoreq. 0.05) the conception rate (63.6%) and 66.0% for groups B and C vs. 50% for group A). The 4 h-increase in the duration of contact of spermatozoa with glycerol before freezing (differentiating technique B from C) did not significantly affect the fertility rate obtained after artificial insemination. On an average, 60.3% of the sows were pregnant with 8.3 embryos.

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