QUANTITATIVE-ANALYSIS OF THE TIME-DEPENDENT DEVELOPMENT OF GLUCOSE-6-PHOSPHATASE-DEFICIENT FOCI IN THE LIVERS OF MICE TREATED NEONATALLY WITH DIETHYLNITROSAMINE
- 1 January 1981
- journal article
- research article
- Vol. 41 (5) , 1585-1593
Abstract
The development of glucose-6-phosphatase (G-6-Pase)-deficient hyperbasophilic foci was analyzed at 4 wk intervals in the livers of CD-1 and C57BL/6J .times. C3H/HeJ F1 (hereafter called B6C3F1) mice given a single i.p. injection of diethylnitrosamine (DEN) (0.1, 0.2 or 0.4 .mu.mol/g body weight) within 24 h after birth. Transections of G-6-Pase-deficient foci of hepatocytes were readily discernible in liver section of DEN-treated mice of either sex at 8 wk of age. The size and number of these foci liver increased with time. The occurrence of G-6-Pase-deficient focus transections with diameters as large as 1 mm coincided with the gross appearance of 1 mm gray-white nodules in the livers of male B6C3F1 mice at 16 wk of age and in females at 32 wk of age. Transections of all grossly visible hepatic nodules from male and female mice were G-6-Pase deficient and hyperbasophilic; the great majority were diagnosed as mouse hepatomas type A. After a single neonatal dose of DEN, the number and rate of growth of the G-6-Pase-deficient foci and the incidence and rate of appearance of gross hepatomas were greater in the livers of male than in those of female mice. The average numbers of G-6-Pase-deficient foci in the livers of male and androgenized female B6C3F1 mice at 36 wk of age were approximately equal and about twice that observed for the livers of DEN-treated female controls. Quantitation of carcinogen-induced histochemically detectable foci and hepatomas as a function of time provides a useful tool for the analysis of initiation and promotion in the mouse liver.This publication has 14 references indexed in Scilit:
- SENSITIVITY AND HETEROGENEITY OF HISTOCHEMICAL MARKERS FOR ALTERED FOCI INVOLVED IN LIVER CARCINOGENESIS1979
- RESISTANCE OF SPONTANEOUS MOUSE HEPATOCELLULAR NEOPLASMS TO IRON ACCUMULATION DURING RAPID IRON LOADING BY PARENTERAL ADMINISTRATION AND THEIR TRANSPLANTABILITY1979
- Enhancement of Rat Hepatocellular-Altered Foci by the Liver Tumor Promoter Phenobarbital: Evidence That Foci Are Precursors of Neoplasms and That the Promoter Acts on Carcinogen-Induced Lesions23JNCI Journal of the National Cancer Institute, 1978
- ENHANCING EFFECT OF PHENOBARBITAL ON DEVELOPMENT OF ENZYME-ALTERED ISLANDS AND HEPATOCELLULAR CARCINOMAS INITIATED BY 3'-METHYL-4-(DIMETHYLAMINO)AZOBENZENE OR DIETHYLNITROSAMINE1978
- RAPID EMERGENCE OF CARCINOGEN-INDUCED HYPERPLASTIC LESIONS IN A NEW MODEL FOR SEQUENTIAL-ANALYSIS OF LIVER CARCINOGENESIS1977
- EFFECTS OF VARYING ONSET AND DURATION OF EXPOSURE TO PHENOBARBITAL ON ITS ENHANCEMENT OF 2-ACETYLAMINOFLUORENE-INDUCED HEPATIC TUMORIGENESIS1977
- Hepatocarcinogenicity of Estragole (1-Allyl-4-methoxybenzene) and 1′-Hydroxyestragole in the Mouse and Mutagenicity of 1′-Acetoxyestragole in Bacteria 2JNCI Journal of the National Cancer Institute, 1976
- STANDARDIZED NOMENCLATURE FOR INBRED STRAINS OF MICE - 6TH LISTING1976
- Development of Preneoplastic and Neoplastic Lesions of the Liver in Male Rats Given 0.025 Percent N-2-Fluorenyldiacetamide2JNCI Journal of the National Cancer Institute, 1965
- LIVER TUMOR INDUCTION BY A SINGLE INJECTION OF O-AMINOAZOTOLUENE TO NEWBORN MICE1965