THE FORM OF LEGUME NITROGEN ASSIMILATED BY NON-LEGUMES WHEN GROWN IN ASSOCIATION1

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Abstract
Soybeans and wheat were grown alone and in association in 2 types of soil, and soybeans alone in N-free sand. Analyses were made on the tops, roots, nodules, and soil or N-free sand for ammonia, nitrites, nitrates, amino acid, and total N at 2 stages of growth, i.e., when the first blooms appeared and when the first pods were about half mature. Ammonia was found in all samples of plants, soil, and N-free sand. The tops and roots of wheat grown in association with soybeans contained a higher ammonia content than those of corresponding wheat plants grown alone. Roots of soybeans grown with wheat contained a higher ammonia content than those of corresponding soybeans grown alone. The tops of soybeans grown alone contained more ammonia than those of corresponding soybean plants grown with wheat. There appeared to be no regularity in the presence or absence of nitrites in the tops, roots, nodules, soil or N-free sand. Nitrates were not found in any of the nodule samples and only irregularly in the tops, roots, and soil or N-free sand. Amino acid N was absent from all soil and N-free sand samples, but was present in all samples of soybean tops. It occurred only occasionally in the tops and roots of wheat and in the roots and nodules of soybeans.

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