Immunological Cross-Reactivity of Type I–III Intermediate Filaments in the Common Carp: In Situ Localization with Use of Heterologous Antibodies
- 1 November 1997
- journal article
- Published by Oxford University Press (OUP) in Transactions of the American Fisheries Society
- Vol. 126 (6) , 948-960
- https://doi.org/10.1577/1548-8659(1997)126<0948:icroti>2.3.co;2
Abstract
The intermediate filaments (IFs) are a multigenic family of 10-nm cytoskeletal polypeptides that have been partially classified according to their cell-specific expression patterns in mammals. Since the IFs have been highly conserved during vertebrate evolution, the objective of the present study was to evaluate the immunological cross-reactivity and tissue distribution patterns of IF types I, II, and III in the common carp Cyprinus carpio. A panel of six heterologous antibodies were evaluated with a streptavidin–biotin–peroxidase complex detection system. The monoclonal antibody AE3, specific for human cytokeratins 1–8 (type II IFs), stained a wide variety of epithelial and nonepithelial tissues. Staining with the AEI monoclonal antibody, specific for human cytokeratins 10, 14–16, and 19 (type 1 IFs), resulted in similar, although generally less intense, staining of all tissues relative to the AE3 antibody. However, the AE1 antibody stained myocardial and skeletal muscle fibers in contrast to th...Keywords
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