DIFFERENTIATION BY ELECTROPHORESIS AND ENZYMO-SEROLOGY OF PROTEINASES PRODUCED BY STRAINS OF STAPHYLOCOCCUS-EPIDERMIDIS BIOTYPE-1 AND BIOTYPE-4

Google Scholar
  • 1 January 1978
    • journal article
    • research article
    • Vol. 8  (1) , 42-49
Abstract
Electrophoretic and serologic characterization of extracellular proteinases from 111 isolates (80 biotype 1 and 32 biotype 4) of S. epidermidis (coagulase negative) of human origin were conducted. Seventy-seven (60 of biotype 1, 17 of biotype 4) of the 111 proteinase-positive isolates of S. epidermidis were classified by serological differentiation of their proteolytic enzymes with known specific antisera. Thirty-two (18 of biotype 1, 14 of biotype 4) isolates of S. epidermidis produced proteolytic enzymes that did not react with any of the known group antisera and were classified as NR (non-reacting). No classification of the enzymes of 2 other isolates was possible. The NR proteinase enzymes from the 32 cultures that could not be classified by serological differentiation with the known specific antisera fitted into 1 of 3 electrophoretic mobility patterns: NR1, NR2 or NR3. Seventy-eight isolates of S. epidermidis biotype 1 were placed into 4 proteinase sero-groups; 49 in F, 11 in B, 11 in NR2 and 7 in NR3. Thirty-one isolates of S. epidermidis biotype 4 were placed into 1 of 2 groups: 17 in C and 14 in NR. All enzymes tested migrated towards the anode at pH 8.6, and the order of electrophoretic mobility was NR3 > F > NR2 > NR1 > B > C. [The presence of these proteinases is directly related to pathogenicity].

This publication has 1 reference indexed in Scilit: