Cu,Zn‐superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis

27 February 2003

journal article
Published by Wiley in FEBS Letters

Vol. 539 (1-3) , 68-72
https://doi.org/10.1016/s0014-5793(03)00199-6

Abstract

Here we analyzed the role of the antioxidant response in Saccharomyces cerevisiae adaptation to hyperosmotic stress. We show that Cu,Zn‐superoxide dismutase (SOD1) plays a fundamental role in this adaptation process since under hyperosmosis SOD1 mutants lead to high protein oxidation levels and show a sensitive phenotype, which is reversed by the addition of N‐acetylcysteine to the medium. Pretreatment with MnCl₂, a superoxide scavenger, improves the survival of the sod1 strain upon hyperosmosis. Additionally, we show that upon hyperosmotic shock there is a small and transient increase in SOD1 transcript levels, regulated by the protein kinase A‐cAMP and SKN7 pathways.

Keywords

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