The Mitotic Apparatus with Unusually Many Microtubules from Sea Urchin Eggs Treated by Hexyleneglycol

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Abstract
The effect of hexyleneglycol on the structure of the mitotic apparatus was studied by light and electron microscopies. By treating sea urchin eggs at prometaphase and metaphase with hexyleneglycol, the mitotic apparatus was found to become remarkably decorated with unusually many astral microtubules which were conspicuously uniform in length. These microtubules appeared to be associated with the granular materials which are most likely microtubule initiating sites or microtubule‐organizing centers.