Identification of Fusarium oxysporum f. sp. cubense Race 4 from Soil or Host Tissue by Cultural Characters

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Abstract
F. oxysporum f. sp. cubense race 4 formed laciniated colonies distinct from those of races 1 and 2 on modified Komada''s medium (K2 medium) but not on potato-dextrose agar, PCNB [pentachloronitrobenzene] agar, Martin''s medium, or surfactant agar. Race 4 was detectable by the laciniated colonies recovered from infected host tissue, and also directly from soil on dilution plates. All isolates of F. oxysporum obtained from 55 wilted ''Cavendish'' banana plants susceptible only to race 4 formed laciniated colonies on K2 medium. None of the isolates obtained from 16 wilted ''Latundan'' banana plants susceptible only to race 1 formed laciniated colonies on the same medium. Six formae speciales of F. oxysporum, 1 saprophytic F. oxysporum and 3 other spp. of Fusarium [F. moniliforme, F. roseum, F. solani] tested did not form laciniated colonies on K2 medium. The recovery of race 4 from experimentally infested soil was about 90% using K2 medium. The population of race 4 in naturally infested soil, determined with K2 medium, ranged 50-650 propagules/g of air-dried soil.

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