PARTIAL CHARACTERIZATION OF A T-CELL-DERIVED FACTOR THAT SUPPRESSES INITIATION OF HUMORAL IMMUNE-RESPONSE INVITRO

Google Scholar
  • 1 January 1977
    • journal article
    • research article
    • Vol. 33  (5) , 745-755
Abstract
Specific anamnestic stimulation of spleen cells from mice immunized 7 days earlier with horse erythrocytes (HRBC) generated the release of a soluble factor that was capable of suppressing the initiation of the in vitro primary .gamma.M immune response to sheep red blood cells (SRBC), as well as to the immunogen that elicited its formation. Moreover, the suppressive macromolecule (MW .apprx. 34,000), derived from antigen-activated, HRBC-primed T [thymus-derived] lymphocytes (but not B [bone marrow-derived] cells), inhibited the secondary .gamma.M and .gamma.G anti-SRBC plaque-forming cell responses of SRBC-primed spleen cells. The active material was resistant to treatment with DNase and RNase, but was inactivated by protease (10 .mu.g/ml, 30 min) or exposure to mild heat (56.degree., 30 min). The antibody initiation suppressor factor (AISF) was concentrated and partially purified by gel filtration, followed by polyacrylamide gel electrophoresis.