The gene encoding ARS-binding factor I is essential for the viability of yeast.
Open Access
- 1 December 1989
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 3 (12a) , 1926-1939
- https://doi.org/10.1101/gad.3.12a.1926
Abstract
The gene encoding a yeast ARS-binding protein, ABF I, has been cloned by screening a genomic lambda gt11 library using monoclonal and polyclonal antibodies against ABF I. ABF I is of interest because it not only binds to ARSs but also to the 5'-flanking region of genes encoding proteins involved in transcription, translation, respiration, and cell-cycle control. The cloned gene has been used to prepare null mutants, which further demonstrate the importance of the ABF I protein by showing that it is essential for vegetative growth. ABF1 maps to chromosome V. The DNA sequence of the ABF1 gene reveals several motifs characteristic of DNA-binding proteins but shows no overall similarity to any protein of known function.This publication has 60 references indexed in Scilit:
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