Isolation of a mouse MT2‐MMP gene from a lung cDNA library and identification of its product
Open Access
- 14 January 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 402 (2-3) , 219-222
- https://doi.org/10.1016/s0014-5793(96)01537-2
Abstract
We have isolated a new MT‐MMP related gene of 3.3 kb from a mouse lung cDNA library using a human MT1‐MMP cDNA as a probe. The deduced protein sequence shows 87% homology to human MT2‐MMP and 52, 50 and 29% to MT1‐MMP, MT3‐MMP and MT4‐MMP, respectively. Thus the gene is thought to be a mouse homologue of human MT2‐MMP. A monoclonal antibody raised against a synthetic peptide recognized mouse MT2‐MMP as a 70 kDa protein. Like MT1‐ and MT3‐MMPs, mouse MT2‐MMP caused activation of pro‐gelatinase A upon co‐transfection into COS‐1 cells. © 1997 Federation of European Biochemical Societies. All rights reservedKeywords
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