Triazine dyes, a new class of affinity labels for nucleotide-dependent enzymes
- 1 October 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 191 (1) , 247-251
- https://doi.org/10.1042/bj1910247
Abstract
A number of reactive dichlorotriazine dyes specifically and irreversibly inactivate pig heart lactate dehydrogenase, yeast glucose 6-phosphate dehydrogenase and yeast hexokinase at sites competitive with NAD+, NADP+, and ATP respectively. Monochlorotriazine dyes, including Cibacron Blue F3G-A, do not inactivate lactate dehydrogenase but display high affinity and thus inhibit the inactivation by dichlorotriazine dyes. These data are interpreted in terms of the ability of nucleotide-binding enzymes to bind polysulphonated aromatic chromophores.This publication has 12 references indexed in Scilit:
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