Abstract
The following N-(.alpha.-hydroxyalkanoyl) derivatives of Leu-Val-Phe-OCH3 leucylvalylphenylalanine methyl ester were synthesized and tested for their ability to inhibit human amniotic renin: D- and L-.alpha.-hydroxyisocaproyl-Leu-Val-Phe-OCH3, D- and L-.alpha.-hydroxyisovaleryl-Leu-Val-Phe-OCH3, L-2-hydroxy-3-phenylpropanoyl-Leu-Val-Phe-OCH3, and D- and L-.alpha.-hydroxyphenylacetyl-Leu-Val-Phe-OCH3. Analysis of the compounds through the use of Dixon plots showed all of the compounds to be competitive inhibitors of renin. All but D-.alpha.-hydroxyisovaleryl-Leu-Val-Phe-OCH3 were more active than the known tetrapeptide inhibitor Leu-Leu-Val-Phe-OCH3. The 2 most active compounds of the series were L-.alpha.-hydroxyisocaproyl-Leu-Val-Phe-OCH3 (Ki [inhibition constant] = 0.23 mM) and L-.alpha.-hydroxyisovaleryl-Leu-Val-Phe-OCH3 (Ki = 0.3 mM).

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