Cytogenetic evidence for hemizygosity at the thymidine kinase locus in P388 mouse lymphoma cells

1 May 1983

journal article
research article
Published by Springer Nature in Cellular and Molecular Life Sciences

Vol. 39 (5) , 532-534
https://doi.org/10.1007/bf01965192

Abstract

Summary A detailed cytogenetic investigation was carried out on P388 mouse lymphoma cells. The cells have a mean chromosome number of 36.86 with a mode and median of 37 chromosomes. G-banding analysis of 12 spreads revealed a total of 15 marker chromosomes with chromosome 11, the determinant of thymidine kinase, being present only in single copy per cell. It is therefore concluded that the P388 cell line is hemizygous at the thymidine kinase locus. Thymidine kinase activities were assayed in P388 cells and two other malignant cell lines, clone 707 Friend mouse leukaemia cells and L5178Y mouse lymphoma cells. No clear relationship was observed between enzyme activity and gene dosage.

Keywords

This publication has 14 references indexed in Scilit:

Increased sensitivity to cell killing and mutagenesis by chemical mutagens in thymidine-kinase-deficient subclones of a Friend murine leukaemia cell line
Genetics Research, 1982
UV sensitivity in thymidine kinase deficient mouse erythroleukaemia cells
Cell Biology International Reports, 1981
The thymidine-kinase locus of friend erythroleukaemic cells
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1981
Synteny of the genes for thymidine kinase and galactokinase in the mouse and their assignment to mouse chromosome 11
Cytogenetic and Genome Research, 1976
Characteristics of spontaneous and induced thymidine and 5-iodo-2-deoxyuridine resistant clones of mouse lymphoma cells
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
The induction of thymidine- and IUdR-resistant variants in P388 mouse lymphoma cells by x-rays, UV and mono- and bi- functional alkylating agents
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
A mutational assay system using the thymidine kinase locus in mouse lymphoma cells
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
Continuous Irradiation of a Murine Lymphoma Line P388Fin Vitro
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1967
Studies on thymidine kinase in cultured mouse fibroblasts
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
STUDIES OF THE CULTURE OF LEUKEMIC CELLS IN VITRO*
Annals of the New York Academy of Sciences, 1958