RADIOIMMUNOASSAY METHOD FOR 1-BETA-D-ARABINOFURANOSYLURACIL USING ANTIBODIES DIRECTED AGAINST 1-BETA-D-ARABINOFURANOSYLCYTOSINE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (2) , 625-628
Abstract
Above pH 7.0 the antitumor agent 1-.beta.-D-arabinofuranosyluracil (ara-U) shows marked pH-dependent cross reactivity with [rabbit] antibodies directed towards 1-.beta.-D-arabinofuranosylcytosine. Since this peculiar phenomenon was not observed with other nucleosides and nucleotides thus far tested, it is probably the result of base catalyzed tautomerism of ara-U to its enolic form which renders it more structurally similar to 1-.beta.-D-arabinofuranosylcytosine. By performing the radioimmunoassay at pH 6.2 and 8.6, 1-.beta.-D-arabinofuranosylcytosine and ara-U could be determined simultaneously. This method for ara-U assay is simple, fairly reliable and applicable to blood level studies.

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