The Stereochemistry of the Reduction of Carbon–Carbon Double Bond with the Cultured Cells of Nicotiana tabacum

Abstract
The stereochemistry in the reduction of the C–C double bond adjacent to the carbonyl group with the cultured cells of Nicotiana tabacum was investigated by feeding (4R)-[6-2H]-(−)-carvone to the cultured cells. It was found that the hydrogen attack to the C–C double bond takes place stereospecifically from the si-face at C-1 and the re-face at C-6, being trans-addition.