Isolation and partial characterization of metaphase chromosomes of a mouse ascites tumor.
- 1 March 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 55 (3) , 642-649
- https://doi.org/10.1073/pnas.55.3.642
Abstract
A method for the isolation of metaphase chromosomes from colchicine-arrested cells of L2 mouse ascites tumor was developed. The unique feature of the isolation procedure was homogenization of cells at pH 3.7. The chromosomes, isolated in mg quantities, were morphologically intact. There was little gel formation on contamination from cells, nuclei, smaller organelles, or soluble components. Chemical analysis of isolated chromosomes showed 13.5% RNA, 13.5% DNA, and 68.3% protein. The ultraviolet absorption spectrum and metal content of chromosomes were presented.This publication has 11 references indexed in Scilit:
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