Blastogenic Transformation Assay for Detection of Immunologic Reactivity to Avian Oncornaviruses2

Abstract
The leukocytes of chickens immunized with live Rous-associated viruses (RAV), RAV-1 or RAY-6, responded in an in vitro leukocyte blastogenic transformation assay when stimulated with either the homologous or heterologous viruses as antigens. This phenomenon appeared related to a cellular response to a common or cross-reacting cell surface antigen induced by several virus subgroups of the avian leukosis-sarcoma complex and seemed to correlate well with other recent in vivo and in vitro studies.

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