Halothane Effect on Beta-Adrenergic Receptors in Canine Myocardium

Publisher Website
Google Scholar
Abstract
Ase activity by binding to myocardial beta-adrenergic receptors, the effect of halothane on binding to these receptors was studied to determine whether this was a site of halothane effect. Beta-adrenergic binding was measured at binding equilibrium in vitro in a canine myocardial membrane preparation in the absence and presence of halothane, 3 to 5 vol%, using as the radioligand 3H-dihydroalprenolol (3H-DHA), a beta-adrenergic antagonist with high affinity and radioactivity. In addition, the effect of halothane on the binding of /-isoproterenol, a beta-adrenergic agonist, was measured by displacement of 3H-DHA. The results indicate that halothane has no effect on either the affinity of canine myocardial beta-adrenergic receptors for 3H-DHA or /-isoproterenol, nor does it alter the number of available receptors at binding equilibrium. Supported in part by a grant from the American Society of Anesthesiologists/Parker B. Francis Foundation. Reprint requests to Dr. Bernstein. Accepted for publication February 9, 1981. © 1981 International Anesthesia Research Society...