ENTEROTOXICITY OF AEROMONAS HYDROPHILA - SKIN-RESPONSES AND INVIVO NEUTRALIZATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 242  (4) , 487-499
Abstract
Culture filtrates and partially purified enterotoxins of 6 strains of A. hydrophila isolated from feces of diarrheic and healthy persons and drinking water, sewage and feces of domestic animals caused induration and increased capillary permeability in skin of adult albino rabbits. The activities were less in crude enterotoxins indicating partial loss during purification. Heat treatment for 30 min at 60.degree. C and 56.degree. C inactivated the induration and permeability effects of culture filtrates and crude enterotoxins, respectively. The activities were most pronounced at pH 8.0 showing gradual loss of activities at lower pH values disappearing completely in culture filtrates at 3.0 and in crude toxins at 4.0. The induration and permeability factor activities of crude enterotoxin were neutralized proportionally in vivo with antitoxin raised against the same in rabbits. Suppression effect was more when antitoxin was infected prior to inoculation of toxin. The assay of the activity of skin permeability factor, being an easier process than loop test, may be used for assay of enterotoxin of A. hydrophila.

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