Dynamics of nuclear actin bundle induction by dimethyl sulfoxide and factors affecting its development.

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Abstract
Dimethyl sulfoxide (DMSO) induced the formation of huge bundles of actin filaments in the nuclei of Dictyostelium mucoroides. The focus is on providing EM data on the function of nuclear actin bundles, illustrating both their formation and their reversion, and on the effects of various factors on the induction. The large nuclear bundles of actin appeared after 20-30 min of treatment with 10% DMSO. A DMSO concentration of 5 or 10% was optimal for the induction of the bundles. The nuclear actin bundle reverted to the original morphology within 5 min after removing DMSO. Induction of nuclear actin bundles was inhibited by Mg2+ and low temperatures, but not by Na+, K+, Ca2+, ATP, cyclic AMP, phosphate buffer, or cytochalasin B. Neither NaN3 nor cycloheximide totally inhibited the induction of the bundles.