Photoreactivation and dark repair of ultraviolet light-induced pyrimidine dimers in chloroplast DNA
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (8) , 2893-2902
- https://doi.org/10.1093/nar/4.8.2893
Abstract
A UV-specific endonuclease was used to detect ultraviolet light-induced pyrimidine dimers in chloroplast DNA of Chlamydomonas reinhardi that was specifically labeled with tritiated thymidine. All of the dimers induced by 100 J/m2 of 254 nm light are removed by photoreaction. Wild-type cells exposed to 50 J/m2 of UF light removed over 80% of the dimers from chloroplast DNA after 24 h of incubation in growth medium in the dark. A UV- sensitive mutant, UVS1, defective in the excision of pyrimidine dimers from nuclear DNA is capable of removing pyrimidine dimers from chloroplast DNA nearly as well as wild-type, suggesting that nuclear and chloroplast DNA dark-repair systems are under separate genetic control.Keywords
This publication has 12 references indexed in Scilit:
- REPAIR OF PYRIMIDINE DIMERS IN ULTRAVIOLET‐IRRADIATED CHLAM YDOMONASPhotochemistry and Photobiology, 1977
- The fate of ultraviolet-induced pyrimidine dimers in the mitochondrial DNA of Saccharomyces cerevisiae following various post-irradiation cell treatmentsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Persistence of pyrimidine dimers during post-replication repair in ultraviolet light-irradiated Escherichia coli K12Journal of Molecular Biology, 1974
- The Absence of a Pyrimidine Dimer Repair Mechanism in Mammalian MitochondriaProceedings of the National Academy of Sciences, 1974
- Absence of ultraviolet-stimulated repair replication in the nuclear and chloroplast genomes of Chlamydomonas reinhardtiBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- IN VIVO SPECIFIC LABELING OF CHLAMYDOMONAS CHLOROPLAST DNAThe Journal of cell biology, 1972
- Synthesis and turnover of Euglena gracilis nuclear and chloroplast deoxyribonucleic acidBiochemistry, 1972
- Physical conservation of parental cytoplasmic DNA through meiosis in Chlamydomonas reinhardi.Proceedings of the National Academy of Sciences, 1968
- Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.Proceedings of the National Academy of Sciences, 1965
- Studies of chloroplast development in Euglena I. Inactivation of green colony formation by U.V. lightBiochimica et Biophysica Acta, 1961