Purification of the β subunit of the fibronectin receptor
- 3 March 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 172 (3) , 713-718
- https://doi.org/10.1111/j.1432-1033.1988.tb13947.x
Abstract
In this work we describe a method for purification of the .beta. subunit of the mouse fibronectin receptor (GP135). Cellular glycoproteins were isolated from a detergent extract of SR-Balb tumor cell membranes by two steps of affinity chromatography on lentil lectin-Sepharose and wheat-germ-agglutinin-agarose. This material was subsequently bound to an Affi gel 102 column and eluted by increasing salt concentration. Most of the GP135 was eluted at 80 mM sodium chloride together with a few other components. A final step of hydroxyapatite chromatography in sodium dodecyl sulphate allowed elution of GP135 as a single chromatographic peak. Fractions containing GP135 were identified at each chromatographic step by immunoblotting with a specific antiserum. By this procedure GP135 was purified to homogeneity as judged by SDS-PAGE analysis of 125I-labelled material.This publication has 27 references indexed in Scilit:
- Amino acid sequence of the human fibronectin receptor.The Journal of cell biology, 1987
- Integrins: A family of cell surface receptorsCell, 1987
- A 135000 molecular weight plasma membrane glycoprotein involved in fibronectin-mediated cell adhesionExperimental Cell Research, 1986
- Selective Inhibition of Fibronectin-Mediated Cell Adhesion by Monoclonal Antibodies to a Cell-Surface GlycoproteinScience, 1985
- Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptorCell, 1985
- Monoclonal Antibodies Which Alter the Morphology of Cultured Chick Myogenic CellsJournal of Cellular Biochemistry, 1982
- Identification and partial characterization of five major membrane glycoproteins of BHK fibroblastsThe Journal of Membrane Biology, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970