The development of Haemonchus contortus in vitro: II. The effect of disulphide-reducing and sulphydryl-blocking reagents on the rate of development to the fourth-stage larvae

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Abstract
Cysteine (20–100 mM) and reduced glutathione (20–80 mM) potentiated the development of the exsheathed third-stage larva of Haemonchus contortus to the fourth stage. The potentiating effect of cysteine was greatest during the short period before and after the exsheathment of the third-stage larva. Two hundred mM cysteine and 1 mM p–chloro-benzoate inhibited development. This inhibition was reversible. Ten mM iodoacetate and 0·5 and 5 mM n–ethyl-maleimide inhibited development of the larvae. The larvae appeared to be most susceptible to the inhibiting effects of these reagents during the period of development approaching the third moult. Development was either little affected or potentiated, when larvae were incubated with these reagents for short periods immediately after exsheathment.