Ultradeep Pyrosequencing Detects Complex Patterns of CD8 + T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques
Open Access
- 15 August 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 83 (16) , 8247-8253
- https://doi.org/10.1128/jvi.00897-09
Abstract
Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8 + T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.This publication has 24 references indexed in Scilit:
- Mauritian Cynomolgus Macaques Share Two Exceptionally Common Major Histocompatibility Complex Class I Alleles That Restrict Simian Immunodeficiency Virus-Specific CD8+T CellsJournal of Virology, 2009
- Adaptation of HIV-1 to human leukocyte antigen class INature, 2009
- Challenges in the development of an HIV-1 vaccineNature, 2008
- Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infectionProceedings of the National Academy of Sciences, 2008
- Vaccination and Timing Influence SIV Immune Escape Viral Dynamics In VivoPLoS Pathogens, 2008
- Estimating the Effectiveness of Simian Immunodeficiency Virus-Specific CD8+T Cells from the Dynamics of Viral Immune EscapeJournal of Virology, 2007
- Characterization of mutation spectra with ultra-deep pyrosequencing: Application to HIV-1 drug resistanceGenome Research, 2007
- The Antiviral Efficacy of Simian Immunodeficiency Virus-Specific CD8 + T Cells Is Unrelated to Epitope Specificity and Is Abrogated by Viral EscapeJournal of Virology, 2007
- Compensatory Substitutions Restore Normal Core Assembly in Simian Immunodeficiency Virus Isolates with Gag Epitope Cytotoxic T-Lymphocyte Escape MutationsJournal of Virology, 2006
- Use of Molecular Beacons for Rapid, Real-Time, Quantitative Monitoring of Cytotoxic T-Lymphocyte Epitope Mutations in Simian Immunodeficiency VirusJournal of Clinical Microbiology, 2005