Purification and characterization of an oxygen-labile, NAD-dependent alcohol dehydrogenase from Desulfovibrio gigas
- 1 May 1993
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 175 (10) , 2859-2863
- https://doi.org/10.1128/jb.175.10.2859-2863.1993
Abstract
A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (Km, 0.15 mM) and 1-propanol (Km, 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.Keywords
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