IMPROVED INSULIN RECEPTOR ASSAY: EFFECTS OF AN ANTIDIABETIC SULPHONYLUREA ON LIVER MEMBRANE INSULIN RECEPTORS FROM OBESE HYPERGLYCAEMIC MICE
Open Access
- 1 June 1979
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 66 (2) , 317-322
- https://doi.org/10.1111/j.1476-5381.1979.tb13682.x
Abstract
1 A simple assay of liver membrane insulin receptors is described, under conditions which apparently stabilized the insulin-receptor complex. The receptor population was measured in the presence of physiological doses of radioactive hormone under conditions which minimized negative co-operativity effects. 2 Equilibrium between receptor and hormone was attained in vitro within 60 min of incubation time at 13°C with no apparent loss of binding sites after 3 h of incubation. 3 Liver membranes from lean mice possessed 8.8 times the receptor number per mg membrane protein measured in membranes from obese hyperglycaemic (ob/ob) littermates. After treatment of ob/ob mice with ARDF-26 (Gliquidon; Boehringer Ingelheim Ltd) the concentration of liver membrane insulin receptors was increased 5 fold.Keywords
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