A comparative study of leucokinin-immunoreactive neurons in insects
- 1 April 1994
- journal article
- research article
- Published by Springer Nature in Cell and tissue research
- Vol. 276 (1) , 69-83
- https://doi.org/10.1007/bf00354786
Abstract
Antisera were raised against leucokinin IV, a member of the leucokinin peptide family. Immunohistochemical localization of leucokinin immunoreactivity in the brain of the cockroach Nauphoeta cinerea revealed neurosecretory cells in the pars intercerebralis and pars lateralis, several bilateral pairs of interneurons in the protocerebrum, and a group of interneurons in the optic lobe. Several immunoreactive interneurons were found in the thoracic ganglia, while the abdominal ganglia contained prominent immunoreactive neurosecretory cells, which projected to the lateral cardiac nerve. The presence of leucokinins in the abdominal nerve cord was confirmed by HPLC combined with ELISA. Leucokinin-immunoreactive neurosecretory cells were also found in the pars intercerebralis of the cricket Acheta domesticus and the mosquito Aedes aegypti, but not in the locust Schistocerca americana or the honey bee Apis mellifera. However, all these species have leucokinin-immunoreactive neurosecretory cells in the abdominal ganglia. The neurohemal organs innervated by abdominal leucokinin-immunoreactive cells were different in each species.Keywords
This publication has 40 references indexed in Scilit:
- Vasopressin-immunoreactive neurons and neurohemal systems in cockroaches and mantidsJournal of Comparative Neurology, 1992
- Isolation and identification of a new diuretic peptide from the tobacco hornworm, Manduca sextaBiochemical and Biophysical Research Communications, 1991
- Presence of corazonin in three insect species, and isolation and identification of [His7]corazonin from Schistocerca americanaPeptides, 1991
- Identification of a diuretic hormone of Locusta migratoriaBiochemical and Biophysical Research Communications, 1991
- A general method for highly selective crosslinking of unprotected polypeptides via pH-controlled modification of N-terminal .alpha.-amino groupsBioconjugate Chemistry, 1990
- Leucokinins, a new family of ion transport stimulators and inhibitors in insect Malpighian tubulesLife Sciences, 1989
- Diversity in neurohaemal organs for homologous neurosecretory cells in different insect species as demonstrated by immunocytochemistry with an antiserum to molluscan cardioexcitatory peptideNeuroscience Letters, 1987
- Juvenile hormone and the development of ovarian responsiveness to a brain hormone in the mosquito, Aedes aegyptiGeneral and Comparative Endocrinology, 1982
- Purification of the two major forms of rat pituitary corticotropin using only reversed-phase liquid chromatographyBiochemistry, 1981
- Can specificity ever be proved in immunocytochemical staining.Journal of Histochemistry & Cytochemistry, 1977