Physiological Effects of Gibberellic Acid. II. On Starch Hydrolyzing Enzymes of Barley Endosperm
Open Access
- 1 November 1960
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 35 (6) , 902-906
- https://doi.org/10.1104/pp.35.6.902
Abstract
Barley grains were dehusked, sterilized with calcium hypochlorite and cut in half transversely. The sterilized endosperm so obtained were treated for 22 hours at 24[degree]C in the presence and absence of 200 [mu]g gibberellic acid (GA), and the amylase activity of the ambient solution was measured and characterized. GA was demonstrated to cause an increase in [alpha]-, and [beta]-amylolytic activity as well as the initiation of activity of a 3d enzyme, similar in some aspects to [alpha]-amylase. It is postulated that a-amylase exists in an inactive state in the endosperm and is not secreted into the endosperm by the embryo. The activation of this and other enzymes during germination is probably dependent upon a series of processes set in motion through the liberation, by the embryo, of a GA-like substance into the endosperm.Keywords
This publication has 7 references indexed in Scilit:
- Physiological Effects of Gibberellic Acid: I. On Carbohydrate Metabolism and Amylase Activity of Barley EndospermPlant Physiology, 1960
- The separation of limit dextrinase from R-enzyme and aspects of the activities of the separated enzymesArchives of Biochemistry and Biophysics, 1959
- Role of Gibberellin-Like Hormones in Regulation of Plant Growth and FloweringNature, 1958
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- Reducing-group production from starch by the action of α- and β-amylases of barley malt. Activity of α- and β-amylasesBiochemical Journal, 1949
- Investigations of Barley and Malt-Amylase.Acta Chemica Scandinavica, 1947
- The beta-amylase of barley.1946