ALKYLATION OF GUANOSINE AND DEOXYGUANOSINE BY PHOSPHORAMIDE MUSTARD
- 1 January 1980
- journal article
- research article
- Vol. 40 (11) , 4183-4186
Abstract
Phosphoramide mustard, an active metabolite of [the antineoplastic drug] cyclophosphamide, was reacted separately with guanosine and deoxyguanosine in aqueous solution at pH 7.4. The major adduct which was formed in each case was isolated by reverse-phase high-pressure liquid chromatography. The structure of the major adduct, as determined by a combination of UV and field desorption mass spectrometry, is that of phosphoramide mustard, one arm of which has reacted with guanosine or deoxyguanosine in position 7. These adducts are much less stable than was 7-methylguanosine; they decompose with a half-life of 2.3 h at 37.degree. and pH 7.4. This instability may contribute to the action of phosphoramide mustard at a molecular level [in animals].This publication has 1 reference indexed in Scilit:
- FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDESBiochemical Journal, 1963