Transport of Phosphate Analogues in Rat-Liver Mitochondria

Publisher Website
Google Scholar
Abstract
The swelling of rat liver mitochondria was studied in isotonic ammonium salts of phosphate, thiophosphate, monofluorphosphate, difluorphosphate or in isotonic ammonium malate induced by low concentrations of these anions. Phosphate, thiophosphate and monofluorphosphate are taken up by the mitochondria, whereas difluorphosphate is not, indicating that the substrate of the transporter represents the divalent anion .**GRAPHIC**. and not .**GRAPHIC**. Thiophosphate, monofluorphosphate and phosphate, but not difluorphosphate induce the swelling of mitochondria in isotonic ammonium malate, only after a lag phase. The length of the lag phase depends on the concentration of inducing anion and pH. This phenomena cannot be explained by the current concept of 2 independent phosphate-transporting systems in mitochondria.