Characterization of Oligodeoxyribonucleotide−Polyethylene Glycol Conjugates by Electrospray Mass Spectrometry

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Abstract
Electrospray mass spectrometry (ESMS) was used to characterize a number of differently functionalized polyethylene glycol−oligodeoxyribonucleotide conjugates. Sample preparation was found to be crucial to obtaining quality data. The resolution and precision of ESMS allowed for the identification of individual conjugates differing in MW by a single ethylene glycol unit with an accuracy of ≤1 (0.02% of the molecular weight). In addition, ESMS was shown to be valuable in identifying chromatographically unresolvable components of a derivatized polyethylene glycol−oligonucleotide conjugate mixture.