Automated Method for the Analysis of Thiamine in Milk, with Application to Other Selected Foods
- 1 September 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 57 (5) , 1081-1084
- https://doi.org/10.1093/jaoac/57.5.1081
Abstract
An automated method for the determination of thiamine in milk and food products is described. The procedure involves the oxidation of thiamine to thiochrome, extraction into isobutanol, and fluorescence measurement. The automated procedure gave a mean recovery value of 92.7% and a standard deviation of ±0.7%, compared to 83.3±2.5% for the manual procedure. The procedure is simple, rapid, and accurate, and markedly shortens the average time for analysis.Keywords
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