Platelet activation by simultaneous actions of diacylglycerol and unsaturated fatty acids.
- 15 July 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (14) , 6443-6446
- https://doi.org/10.1073/pnas.89.14.6443
Abstract
Several cis-unsaturated fatty acids such as oleic, linoleic, linolenic, eicosapentaenoic, and docosahexaenoic acids added directly to intact human platelets greatly enhance protein kinase C activation as judged by the phosphorylation of its specific endogenous substrate, a 47-kDa protein. This enhancement absolutely requires the presence of a membrane-permeant diacylglycerol, 1,2-dioctanoylglycerol, or a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate. In the presence of ionomycin and either 1,2-dioctanoylglycerol or phorbol 12-myristate 13-acetate, the release of serotonin from the platelets is also remarkably increased by cis-unsaturated fatty acids. The effect of these fatty acids is observed at concentrations less than 50 microM. Saturated fatty acids and trans-unsaturated fatty acids are inactive. Titration of ionomycin to induce a release reaction and measurement of the intracellular Ca2+ level by the fura-2 procedure indicate that cis-unsaturated fatty acids increase an apparent sensitivity of the platelet response to Ca2+. The results suggest that cis-unsaturated fatty acids, which are presumably produced from phosphatidylcholine by signal-dependent activation of phospholipase A2, may take part directly in cell signaling through the protein kinase C pathway.Keywords
This publication has 25 references indexed in Scilit:
- Arachidonic acid and diacylglycerol act synergistically to activate protein kinase C in vitro and in vivoBiochemical and Biophysical Research Communications, 1991
- Dual effect of arachidonic acid on protein kinase C isoenzymes isolated from rabbit thymus cellsFEBS Letters, 1990
- Receptor-mediated activation of phospholipase A2 via GTP-binding proteins: arachidonic acid and its metabolites as second messengersTrends in Neurosciences, 1988
- Activation of protein kinase C by cis- and trans-octadecadienoic acids in intact human platelets and its potentiation by diacylglycerolBiochemical and Biophysical Research Communications, 1987
- Three distinct forms of rat brain protein kinase C: Differential response to unsaturated fatty acidsBiochemical and Biophysical Research Communications, 1987
- Direct activation of purified protein kinase C by unsaturated fatty acids (oleate and arachidonate) in the absence of phospholipids and Ca2+FEBS Letters, 1985
- Synergistic functions of phorbol ester and calcium in serotonin release from human plateletsBiochemical and Biophysical Research Communications, 1983
- Phospholipid turnover as a possible transmembrane signal for protein phosphorylation during human platelet activation by thrombinBiochemical and Biophysical Research Communications, 1980
- Relationship between phosphorylation of blood platelet proteins and secretion of platelet granule constituents I. Effects of different aggregating agentsBiochemical and Biophysical Research Communications, 1977
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959