A biotin‐dependent sodium pump: glutaconyl‐CoA decarboxylase from Acidaminococcus fermentans
Open Access
- 1 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 148 (1) , 35-38
- https://doi.org/10.1016/0014-5793(82)81237-4
Abstract
The decarboxylation of glutaconyl‐CoA to crotonyl‐CoA in the anaerobic bacterium Acidaminococcus fermentans is catalysed by a membrane‐bound, biotin‐dependent enzyme which requires Na+ for activity. Inverted vesicles from A. fermentans accumulated Na+ only if glutaconyl‐CoA was decarboxylated. The Na+ uptake was inhibited by avidin but not by the avidin biotin complex. Detergents and ionophores such as monensin also prevented the Na+ transport. The results indicate that the enzyme is able to convert the free energy of decarboxylation (ΔG o′ ≈−30 kJ/mol) into a Na+ gradient.Keywords
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