Microchromatography of hemoglobins. II. A simplified procedure for the determination of hemoglobin A2.

  • 1 October 1975
    • journal article
    • Vol. 86  (4) , 700-2
Abstract
A modification of the microchromatographic procedure for Hb-A2 which utilizes DEAE-cellulose (Reference 1) allows the quantitation of Hb-A2 without interference from any Hb-S in the sample. Elution of Hb-A2 with a glycine-KCN developer is much less sensitive to minor change in pH of the developer, but is greatly dependent on the pH of the ion exchanger.

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