cDNA structure and in situ localization of the Aplysia californica pro‐hormone convertase PC2
- 20 September 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 330 (3) , 343-346
- https://doi.org/10.1016/0014-5793(93)80901-6
Abstract
The complete cDNA structure of the Aplysia californica pro-protein and pro-hormone convertase PC2 (aPC2) was obtained from a cDNA library of the nervous system. The deduced amino acid sequence revealed that aPC2 exhibits an 85%, 61% and 62% sequence identity to the Lymnaea stagnalis, Xenopus laevis and mouse PC2 homologues, respectively. The deduced primary sequence suggested a protein of 653 amino acids which includes a 27- and 88-amino acid signal peptide and pro-segment. The signal peptide and the C-terminal segments are the least conserved regions. On Northern blots of nervous system we detected a transcript of 6.8 kb. The in situ hybridization histochemistry on the abdominal ganglion revealed intense labeling of the bag cells. Large peptidergic cells and clusters of sensory and motor neurons also contained high levels of aPC2 mRNA.Keywords
This publication has 26 references indexed in Scilit:
- Molluscan putative prohormore convertases: Structural diversity in the central nervous system of Lymnaea stagnalisFEBS Letters, 1992
- The cDNA structure of the porcine pro‐hormone convertase PC2 and the comparative processing by PC1 and PC2 of the N‐terminal glycopeptide segment of porcine POMCFEBS Letters, 1992
- Testicular expression of PC4 in the rat: molecular diversity of a novel germ cell-specific Kex2/subtilisin-like proprotein convertaseMolecular Endocrinology, 1992
- Identification and molecular cloning of a neuropeptide y homolog that produces prolonged inhibition in aplysia neuronsNeuron, 1992
- Structure and expression of Xenopus prohormone convertase PC2FEBS Letters, 1992
- Proprotein and prohormone convertases of the subtilisin familyTrends in Endocrinology & Metabolism, 1992
- Identification of a Second Human Subtilisin-Like Protease Gene in thefes/fpsRegion of Chromosome 15DNA and Cell Biology, 1991
- Peptide Processing and Targeting in the Neuronal Secretory PathwayScience, 1991
- cDNA Sequence of Two Distinct Pituitary Proteins Homologous to Kex2 and Furin Gene Products: Tissue-Specific mRNAs Encoding Candidates for Pro-Hormone Processing ProteinasesDNA and Cell Biology, 1990
- Molecular aspects of egg-laying behavior inAplysia californicaBehavior Genetics, 1990